ODONTOGENESIS 2019
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Contents
Preface v
Contributors xi
Part I Protocols for Establishment of Dental Cells Lines and Animal Models
1 Microdissection and Isolation of Mouse Dental Epithelial Cells
of Continuously Growing Mouse Incisors 3
Hidemitsu Harada and Keishi Otsu
2 Establishment of an Immortalized Mouse Bmp2 Knockout Dental
Papilla Mesenchymal Cell Line 13
Wen'an Xu and Shuo Chen
3 Establishment of Stable Cell Lines from Primary Human Dental Pulp
Stem Cells 21
Elizabeth Guirado, Youbin Zhang, and Anne George
4 Isolation of Dental Stem Cell-Enriched Populations from Continuously
Growing Mouse Incisors 29
Anamaria Balic
5 Application of Cell Lineage Tracing Combined with Immunofluorescence
in the Study of Dentinogenesis 39
Yan Jing, Chaoyuan Li, and Jian Q. Feng
6 Tissue Recombination and Kidney Capsule Transplantation Assays
for the Study of Epithelial-Mesenchymal Interactions 49
Lucia Jimenez-Rojo and Thimios A. Mitsiadis
Part II Protocols for Dental Stem Cells and Tissue Regeneration
7 Dental Mesenchymal Stem Cells: Dental Pulp Stem Cells, Periodontal Ligament Stem Cells, Apical Papilla Stem Cells, and Primary Teeth Stem Cells—Isolation, Characterization, and Expansion for Tissue
Engineering 59
Mey Al-Habib and George T. -J. Huang
8 Phenotypic Identification of Dental Pulp Mesenchymal Stem/Stromal
Cells Subpopulations with Multiparametric Flow Cytometry 77
Maxime Ducret, Jean-Christophe Farges, Marielle Pasdeloup,
Emeline Perrier-Groult, Andreas Mueller, Frederic Mallein-Gerin, and Hugo Fabre
9 Dental Pulp Stem Cells: Isolation, Characterization, Expansion,
and Odontoblast Differentiation for Tissue Engineering 91
Qing Dong, Yuanyuan Wang, Fatemeh Mohabatpour, Li Zheng,
Silvana Papagerakis, Daniel Chen, and Petros Papagerakis
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